Parasitology meets cryo-electron tomography - exciting prospects await.
Trends Parasitol
; 38(5): 365-378, 2022 05.
Article
em En
| MEDLINE
| ID: mdl-35148963
ABSTRACT
Cryo-electron tomography (cryo-ET) is a cryo-electron microscopy (EM) approach that allows 3D imaging of cellular structures in near-native, frozen-hydrated conditions with molecular resolution. Continued development of technologies, including direct electron detectors, phase plates, and energy filters, has improved the information yield from cellular samples, which is further extended by newly developed workflows for data collection and analyses. Moreover, advanced sample-thinning techniques, such as cryogenic focused ion-beam (cryo-FIB) milling, provide access to parasitic events and structures that were previously inaccessible for cryo-ET. Cryo-ET has therefore become more versatile and capable of transforming our understanding of parasite biology, particularly that of apicomplexans. This review discusses cryo-ET's implementation, its recent contributions, and how it can reveal pathogenesis mechanisms in the near future using apicomplexans as a case study.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tomografia com Microscopia Eletrônica
Idioma:
En
Revista:
Trends Parasitol
Ano de publicação:
2022
Tipo de documento:
Article