Effects of BCG vaccination on donor unrestricted T cells in two prospective cohort studies.
EBioMedicine
; 76: 103839, 2022 Feb.
Article
em En
| MEDLINE
| ID: mdl-35149285
ABSTRACT
BACKGROUND:
Non-protein antigen classes can be presented to T cells by near-monomorphic antigen-presenting molecules such as CD1, MR1, and butyrophilin 3A1. Such T cells, referred to as donor unrestricted T (DURT) cells, typically express stereotypic T cell receptors. The near-unrestricted nature of DURT cell antigen recognition is of particular interest for vaccine development, and we sought to define the roles of DURT cells, including MR1-restricted MAIT cells, CD1b-restricted glucose monomycolate (GMM)-specific T cells, CD1d-restricted NKT cells, and γδ T cells, in vaccination against Mycobacterium tuberculosis.METHODS:
We compared and characterized DURT cells following primary bacille Calmette-Guerin (BCG) vaccination in a cohort of vaccinated and unvaccinated infants, as well as before and after BCG-revaccination in adults.FINDINGS:
BCG (re)vaccination did not modulate peripheral blood frequencies, T cell activation or memory profiles of MAIT cells, CD1b-restricted GMM-specific and germline-encoded mycolyl-reactive (GEM) cells or CD1d-restricted NKT cells. By contrast, primary BCG vaccination was associated with increased frequencies of γδ T cells as well as a novel subset of CD26+CD161+TRAV1-2- IFN-γ-expressing CD4+ T cells in infants.INTERPRETATION:
Our findings, that most DURT cell populations were not modulated by BCG, do not preclude a role of BCG in modulating other qualitative aspects of DURT cells. More studies are required to understand the full potential of DURT cells in new TB vaccine strategies.FUNDING:
Aeras, the National Institutes of Health, and the Bill and Melinda Gates Foundation.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
2_ODS3
/
3_ND
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4_TD
Base de dados:
MEDLINE
Assunto principal:
Células T Invariantes Associadas à Mucosa
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Mycobacterium tuberculosis
Tipo de estudo:
Etiology_studies
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Observational_studies
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Qualitative_research
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Risk_factors_studies
Limite:
Adult
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Humans
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Infant
Idioma:
En
Revista:
EBioMedicine
Ano de publicação:
2022
Tipo de documento:
Article