Omentin1 induces osteoblast viability and differentiation via the TGFß/Smad signaling pathway in osteoporosis.
Mol Med Rep
; 25(4)2022 04.
Article
em En
| MEDLINE
| ID: mdl-35179221
ABSTRACT
Osteoporosis is a bonerelated disease that results from impaired bone formation and excessive bone resorption. The potential value of adipokines has been investigated previously, due to their influence on osteogenesis. However, the osteogenic effects induced by omentin1 remain unclear. The aim of the present study was to determine the regulatory effects of omentin1 on osteoblast viability and differentiation, as well as to explore the underlying molecular mechanism. The present study investigated the effects of omentin1 on the viability and differentiation of mouse preosteoblast cells (MC3T3E1) using quantitative and qualitative measures. A Cell Counting Kit8 assay was used to assess the viability of MC3T3E1 cells following treatment with different doses of omentin1. Omentin1 and bone morphogenetic protein (BMP) inhibitor were added to osteogenic induction mediums in different ways to assess their effect. The alkaline phosphatase (ALP) activity and Alizarin Red S (ARS) staining of MC3T3E1 cells treated with omentin1 and/or BMP inhibitor were used to examine the effects of omentin1 on differentiation and mineralization. Western blotting was used to further explore its potential mechanism, and to study the role of omentin1 on the viability and differentiation of osteoblasts. The results showed that omentin1 altered the viability of MC3T3E1 cells in a dosedependent manner. Omentin1 treatment significantly increased the expression of members of the TGFß/Smad signaling pathway. In the omentin1 group, the ALP activity of the MC3T3E1 cells was increased, and the ARS staining area was also increased. The mRNA and protein expression levels of BMP2, Runtrelated transcription factor 2, collagen1, osteopontin, osteocalcin and osterix in the omentin1 group were also significantly upregulated. All these effects were reversed following treatment with SIS3 HCl. These results demonstrated that omentin1 can significantly promote osteoblast viability and differentiation via the TGFß/Smad signaling pathway, thereby promoting bone formation and preventing osteoporosis.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osteoblastos
/
Osteoporose
Tipo de estudo:
Qualitative_research
Limite:
Animals
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2022
Tipo de documento:
Article