[Preparation of iduronate sulfatase from the human placenta]. / Preparazione di iduronato solfatasi da placenta umana.
Boll Soc Ital Biol Sper
; 54(17): 1587-91, 1978 Sep 15.
Article
em It
| MEDLINE
| ID: mdl-35184
ABSTRACT
The preparation of the enzyme iduronate sulfatase from human placenta has been undertaken. The substrate O-(alpha-L-idopyranosyluronic acid 2-sulfate) (1 leads to 4)-2,5-anhydro-D-[3H]mannitol 6-sulfate was used to measure the enzymatic activity. The enzyme shows a pH optimum of 4.0 in 0.1 M sodium formiate or acetate buffer. Chromatography on DE-52 gives a 5.4 fold purification. The enzyme is inhibited by NaCl or KCl in 20 mM salt the reaction rate was only 63% and 34% respectively. Inhibition by salt can be removed by extensive dialysis after the chromatographic step.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Placenta
/
Sulfatases
/
Iduronato Sulfatase
Limite:
Humans
Idioma:
It
Revista:
Boll Soc Ital Biol Sper
Ano de publicação:
1978
Tipo de documento:
Article