Intravital Microscopy in Atherosclerosis Research.
Methods Mol Biol
; 2419: 645-658, 2022.
Article
em En
| MEDLINE
| ID: mdl-35237994
ABSTRACT
Atherosclerosis is a lipid-driven inflammatory disorder that narrows the arterial lumen and can induce life-threatening complications from coronary artery disease, cerebrovascular disease, and peripheral artery disease. On a mechanistic level, the development of novel cellular-resolution intravital microscopy imaging approaches has recently enabled in vivo studies of underlying biological processes governing disease onset and progress. In particular, multiphoton microscopy has emerged as a promising intravital imaging tool utilizing two-photon-excited fluorescence and second-harmonic generation that provides subcellular resolution and increased imaging depths beyond confocal and epifluorescence microscopy. In this chapter, we describe the state-of-the-art multiphoton microscopy applied to the study of murine atherosclerosis.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Microscopia de Fluorescência por Excitação Multifotônica
/
Aterosclerose
Limite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2022
Tipo de documento:
Article