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Mass cytometry staining for human bone marrow clinical samples.
Hallisey, Margaret; Dennis, Jenna; Abrecht, Charlotte; Pistofidis, Romanos Sklavenitis; Schork, Abigail N; Lightbody, Elizabeth D; Heilpern-Mallory, Daniel; Severgnini, Mariano; Ghobrial, Irene M; Hodi, F Stephen; Baginska, Joanna.
Afiliação
  • Hallisey M; Department of Medical Oncology, Center for Immuno-Oncology, Dana-Farber Cancer Institute, 450 Brookline Avenue, Boston, MA 02215, USA.
  • Dennis J; Department of Medical Oncology, Center for Immuno-Oncology, Dana-Farber Cancer Institute, 450 Brookline Avenue, Boston, MA 02215, USA.
  • Abrecht C; Department of Medical Oncology, Center for Immuno-Oncology, Dana-Farber Cancer Institute, 450 Brookline Avenue, Boston, MA 02215, USA.
  • Pistofidis RS; Department of Medical Oncology, Center for Immuno-Oncology, Dana-Farber Cancer Institute, 450 Brookline Avenue, Boston, MA 02215, USA.
  • Schork AN; Center for Prevention of Progression of Blood Cancers, Dana-Farber Cancer Institute, 450 Brookline Avenue, Boston, MA 02215, USA.
  • Lightbody ED; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Heilpern-Mallory D; Longwood Medical Area CyTOF Core, Dana-Farber Cancer Institute, Boston, MA 02215, USA.
  • Severgnini M; Department of Medical Oncology, Center for Immuno-Oncology, Dana-Farber Cancer Institute, 450 Brookline Avenue, Boston, MA 02215, USA.
  • Ghobrial IM; Center for Prevention of Progression of Blood Cancers, Dana-Farber Cancer Institute, 450 Brookline Avenue, Boston, MA 02215, USA.
  • Hodi FS; Department of Medical Oncology, Center for Immuno-Oncology, Dana-Farber Cancer Institute, 450 Brookline Avenue, Boston, MA 02215, USA.
  • Baginska J; Center for Prevention of Progression of Blood Cancers, Dana-Farber Cancer Institute, 450 Brookline Avenue, Boston, MA 02215, USA.
STAR Protoc ; 3(1): 101163, 2022 03 18.
Article em En | MEDLINE | ID: mdl-35243367
ABSTRACT
This protocol details a staining technique optimized for immunophenotyping of human bone marrow immune populations using mass cytometry. The protocol accounts for the limitations of working with human bone marrow, such as reduced viability, low cell counts, and fragile cell pellets, to successfully acquire single viable cells ready for downstream analysis. This assay can be used to characterize the activation, exhaustion, and cytotoxicity of immune populations and ensure comprehensive immunophenotyping of human bone marrow clinical samples.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Medula Óssea / Células da Medula Óssea Tipo de estudo: Guideline Limite: Humans Idioma: En Revista: STAR Protoc Ano de publicação: 2022 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Medula Óssea / Células da Medula Óssea Tipo de estudo: Guideline Limite: Humans Idioma: En Revista: STAR Protoc Ano de publicação: 2022 Tipo de documento: Article