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A Dual-Purpose Real-Time Indicator and Transcriptional Integrator for Calcium Detection in Living Cells.
Erdenee, Elbegduuren; Ting, Alice Y.
Afiliação
  • Erdenee E; Department of Biology, Stanford University, Stanford, California 94305, United States.
  • Ting AY; Department of Biology, Stanford University, Stanford, California 94305, United States.
ACS Synth Biol ; 11(3): 1086-1095, 2022 03 18.
Article em En | MEDLINE | ID: mdl-35254056
Calcium is a ubiquitous second messenger in eukaryotes, correlated with neuronal activity and T-cell activation among other processes. Real-time calcium indicators such as GCaMP have recently been complemented by newer calcium integrators that convert transient calcium activity into stable gene expression. Here we introduce LuCID, a dual-purpose real-time calcium indicator and transcriptional calcium integrator that combines the benefits of both calcium detection technologies. We show that the calcium-dependent split luciferase component of LuCID provides a real-time bioluminescence readout of calcium dynamics in cells, while the GI/FKF1 split GAL4 component of LuCID converts calcium-generated bioluminescence into stable gene expression. We also show that LuCID's modular design enables it to read out other cellular events such as protein-protein interactions. LuCID adds to the arsenal of tools for studying cells and cell populations that utilize calcium for signaling.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cálcio / Neurônios Tipo de estudo: Diagnostic_studies Idioma: En Revista: ACS Synth Biol Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cálcio / Neurônios Tipo de estudo: Diagnostic_studies Idioma: En Revista: ACS Synth Biol Ano de publicação: 2022 Tipo de documento: Article