Your browser doesn't support javascript.
loading
GBS-MeDIP: A protocol for parallel identification of genetic and epigenetic variation in the same reduced fraction of genomes across individuals.
Rezaei, Shiva; Uffenorde, Julia; Gimm, Oliver; Hosseinpour Feizi, Mohammad Ali; Miemczyk, Stefan; Coutinho, Luiz Lehmann; Jensen, Per; Guerrero-Bosagna, Carlos; Pértille, Fábio.
Afiliação
  • Rezaei S; Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz 5166616471, Iran.
  • Uffenorde J; Avian Behavioral Genomics and Physiology Group, Department of Physics, Chemistry and Biology (IFM), Linköping University, 581 83 Linköping, Sweden.
  • Gimm O; Department of Surgery and Department of Biomedical and Clinical Sciences (BKV), Linköping University, 581 83 Linköping, Sweden.
  • Hosseinpour Feizi MA; Department of Surgery and Department of Biomedical and Clinical Sciences (BKV), Linköping University, 581 83 Linköping, Sweden.
  • Miemczyk S; Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz 5166616471, Iran.
  • Coutinho LL; Avian Behavioral Genomics and Physiology Group, Department of Physics, Chemistry and Biology (IFM), Linköping University, 581 83 Linköping, Sweden.
  • Jensen P; Environmental Toxicology Program, Department of Organismal Biology, Uppsala University, 752 36 Uppsala, Sweden.
  • Guerrero-Bosagna C; Avian Behavioral Genomics and Physiology Group, Department of Physics, Chemistry and Biology (IFM), Linköping University, 581 83 Linköping, Sweden.
  • Pértille F; Avian Behavioral Genomics and Physiology Group, Department of Physics, Chemistry and Biology (IFM), Linköping University, 581 83 Linköping, Sweden.
STAR Protoc ; 3(1): 101202, 2022 03 18.
Article em En | MEDLINE | ID: mdl-35257114
ABSTRACT
The GBS-MeDIP protocol combines two previously described techniques, Genotype-by-Sequencing (GBS) and Methylated-DNA-Immunoprecipitation (MeDIP). Our method allows for parallel and cost-efficient interrogation of genetic and methylomic variants in the DNA of many reduced genomes, taking advantage of the barcoding of DNA samples performed in the GBS and the subsequent creation of DNA pools, then used as an input for the MeDIP. The GBS-MeDIP is particularly suitable to identify genetic and methylomic biomarkers when resources for whole genome interrogation are lacking.
Assuntos
Palavras-chave
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA / Metilação de DNA Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: STAR Protoc Ano de publicação: 2022 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA / Metilação de DNA Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: STAR Protoc Ano de publicação: 2022 Tipo de documento: Article