Uniform water-mediated saturation transfer: A sensitivity-improved alternative to WaterLOGSY.
J Magn Reson
; 338: 107190, 2022 05.
Article
em En
| MEDLINE
| ID: mdl-35306453
ABSTRACT
In the study of small molecule ligands and candidate macromolecular targets, water spins in long-lived association with macromolecules (proteins or nanoparticles) constitute a remarkable source of magnetization that can be exploited to reveal ligand-target binding. In this work we show how the selective saturation of water spins complemented with adiabatic off-resonance spin-locks can remove the NOE contribution of bulk water in the final difference spectrum, leading to uniformly enhanced signals that reveal weak ligand-target interactions.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Água
/
Nanopartículas
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
J Magn Reson
Ano de publicação:
2022
Tipo de documento:
Article