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Characterization of Shigella flexneri-specific murine monoclonal antibodies by chemically defined glycoconjugates.
J Immunol ; 137(7): 2361-6, 1986 Oct 01.
Article em En | MEDLINE | ID: mdl-3531329
Chemically defined glycoconjugates are demonstrated to have considerable potential for selecting hybridoma antibodies directed toward O-antigenic determinants, especially when used in combination with a panel of well-characterized LPS molecules. Monoclonal antibodies specific for the Shigella flexneri O-antigens of serogroup 5b, variants X and Y, were generated after immunization of BALB/c mice with killed bacterial cells, and active hybrids were selected on the basis of ELISA performed with the purified serotype-specific LPS antigen. Subsequent screening with a variety of glycoconjugates, derived from synthetic oligosaccharides and larger structures obtained by phage Sf6/endo-rhamnosidase hydrolysis of purified LPS established a detailed profile of binding characteristics for Shigella flexneri variant Y-specific antibodies. Together with the results of precipitin analysis and heavy chain isotyping experiments, a limited number of antibodies were selected as candidates for detailed studies of the antibody combining site.
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Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Assunto principal: Polissacarídeos Bacterianos / Shigella flexneri / Anticorpos Monoclonais Limite: Animals Idioma: En Revista: J Immunol Ano de publicação: 1986 Tipo de documento: Article
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Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Assunto principal: Polissacarídeos Bacterianos / Shigella flexneri / Anticorpos Monoclonais Limite: Animals Idioma: En Revista: J Immunol Ano de publicação: 1986 Tipo de documento: Article