Characterization of Shigella flexneri-specific murine monoclonal antibodies by chemically defined glycoconjugates.
J Immunol
; 137(7): 2361-6, 1986 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-3531329
Chemically defined glycoconjugates are demonstrated to have considerable potential for selecting hybridoma antibodies directed toward O-antigenic determinants, especially when used in combination with a panel of well-characterized LPS molecules. Monoclonal antibodies specific for the Shigella flexneri O-antigens of serogroup 5b, variants X and Y, were generated after immunization of BALB/c mice with killed bacterial cells, and active hybrids were selected on the basis of ELISA performed with the purified serotype-specific LPS antigen. Subsequent screening with a variety of glycoconjugates, derived from synthetic oligosaccharides and larger structures obtained by phage Sf6/endo-rhamnosidase hydrolysis of purified LPS established a detailed profile of binding characteristics for Shigella flexneri variant Y-specific antibodies. Together with the results of precipitin analysis and heavy chain isotyping experiments, a limited number of antibodies were selected as candidates for detailed studies of the antibody combining site.
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Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos Bacterianos
/
Shigella flexneri
/
Anticorpos Monoclonais
Limite:
Animals
Idioma:
En
Revista:
J Immunol
Ano de publicação:
1986
Tipo de documento:
Article