Approaches for CRISPR/Cas9 Genome Editing in C. elegans.
Methods Mol Biol
; 2468: 215-237, 2022.
Article
em En
| MEDLINE
| ID: mdl-35320567
ABSTRACT
The clustered, regularly interspaced, short, palindromic repeat (CRISPR)-associated (CAS) nuclease Cas9 has been used in many organisms to generate specific mutations and transgene insertions. Here we describe our most up-to-date protocols using the S. pyogenes Cas9 in C. elegans that provides a convenient and effective approach for making heritable changes to the worm genome. We present several considerations when deciding which strategy best suits the needs of the experiment.
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Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Caenorhabditis elegans
/
Genoma Helmíntico
/
Sistemas CRISPR-Cas
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Edição de Genes
Limite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2022
Tipo de documento:
Article