Expression of the denV gene of coliphage T4 in UV-sensitive rad mutants of Saccharomyces cerevisiae.
Mol Cell Biol
; 6(10): 3559-62, 1986 Oct.
Article
em En
| MEDLINE
| ID: mdl-3540595
ABSTRACT
A plasmid containing the denV gene from bacteriophage T4, under the control of the yeast alcohol dehydrogenase I (ADC1) promoter, conferred a substantial increase in UV resistance in the UV-sensitive Saccharomyces cerevisiae mutants rad1-2 and rad3-2. The UV resistance of the denV+ yeast cells was cell cycle dependent and correlated well with the level of the denV gene product as measured by immunoblotting and by a photoreversal assay for pyrimidine dimer-DNA glycosylase activity.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Fagos T
/
Raios Ultravioleta
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Álcool Desidrogenase
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Escherichia coli
/
Genes
/
Genes Virais
/
Mutação
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Mol Cell Biol
Ano de publicação:
1986
Tipo de documento:
Article