Reactive Acrylamide-Modified DNA Traps for Accurate Cross-Linking with Cysteine Residues in DNA-Protein Complexes Using Mismatch Repair Protein MutS as a Model.

Monakhova, Mayya V; Kubareva, Elena A; Kolesnikov, Kirill K; Anashkin, Viktor A; Kosaretskiy, Egor M; Zvereva, Maria I; Romanova, Elena A; Friedhoff, Peter; Oretskaya, Tatiana S; Zatsepin, Timofei S

Monakhova, Mayya V; Kubareva, Elena A; Kolesnikov, Kirill K; Anashkin, Viktor A; Kosaretskiy, Egor M; Zvereva, Maria I; Romanova, Elena A; Friedhoff, Peter; Oretskaya, Tatiana S; Zatsepin, Timofei S.

Afiliação

Monakhova MV; A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Leninskye Gory 1, 119991 Moscow, Russia.
Kubareva EA; A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Leninskye Gory 1, 119991 Moscow, Russia.
Kolesnikov KK; Department of Chemistry, Lomonosov Moscow State University, Leninskye Gory 1, 119991 Moscow, Russia.
Anashkin VA; A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Leninskye Gory 1, 119991 Moscow, Russia.
Kosaretskiy EM; Department of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Leninskye Gory 1, 119991 Moscow, Russia.
Zvereva MI; Department of Chemistry, Lomonosov Moscow State University, Leninskye Gory 1, 119991 Moscow, Russia.
Romanova EA; A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Leninskye Gory 1, 119991 Moscow, Russia.
Friedhoff P; Institute for Biochemistry, FB 08, Justus Liebig University, Heinrich-Buff-Ring 17, D-35392 Giessen, Germany.
Oretskaya TS; A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Leninskye Gory 1, 119991 Moscow, Russia.
Zatsepin TS; Department of Chemistry, Lomonosov Moscow State University, Leninskye Gory 1, 119991 Moscow, Russia.

Molecules ; 27(8)2022 Apr 10.

Article em En | MEDLINE | ID: mdl-35458636

Assuntos

Cisteína; Proteínas de Escherichia coli; Acrilamida; Pareamento Incorreto de Bases; Cisteína/química; DNA/química; Reparo de Erro de Pareamento de DNA; Reparo do DNA; Proteínas de Escherichia coli/metabolismo; Proteína MutS de Ligação de DNA com Erro de Pareamento/química; Proteína MutS de Ligação de DNA com Erro de Pareamento/metabolismo; Proteínas

Palavras-chave

DNA mismatch repair; DNA modification; DNAprotein complex; MutS; crosslinking; modified oligonucleotide; regioselectivity

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cisteína / Proteínas de Escherichia coli Idioma: En Revista: Molecules Ano de publicação: 2022 Tipo de documento: Article

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