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Tryptophan promoted ß-defensin-2 expression via the mTOR pathway and its metabolites: kynurenine banding to aryl hydrocarbon receptor in rat intestine.
Tang, Zhiru; Shi, Baoshi; Sun, Weizhong; Yin, Yulong; Chen, Qingju; Mohamed, Taha; Lu, Changwen; Sun, Zhihong.
Afiliação
  • Tang Z; Laboratory for Bio-feed and Molecular Nutrition, College of Animal Science and Technology, Southwest University Chongqing 400715 China sunzh2002cn@aliyun.com.
  • Shi B; Laboratory for Bio-feed and Molecular Nutrition, College of Animal Science and Technology, Southwest University Chongqing 400715 China sunzh2002cn@aliyun.com.
  • Sun W; Laboratory for Bio-feed and Molecular Nutrition, College of Animal Science and Technology, Southwest University Chongqing 400715 China sunzh2002cn@aliyun.com.
  • Yin Y; Institute of Subtropical Agriculture, The Chinese Academy of Sciences Changsha 410125 P. R. China.
  • Chen Q; Laboratory for Bio-feed and Molecular Nutrition, College of Animal Science and Technology, Southwest University Chongqing 400715 China sunzh2002cn@aliyun.com.
  • Mohamed T; Laboratory for Bio-feed and Molecular Nutrition, College of Animal Science and Technology, Southwest University Chongqing 400715 China sunzh2002cn@aliyun.com.
  • Lu C; Laboratory for Bio-feed and Molecular Nutrition, College of Animal Science and Technology, Southwest University Chongqing 400715 China sunzh2002cn@aliyun.com.
  • Sun Z; Laboratory for Bio-feed and Molecular Nutrition, College of Animal Science and Technology, Southwest University Chongqing 400715 China sunzh2002cn@aliyun.com.
RSC Adv ; 10(6): 3371-3379, 2020 Jan 16.
Article em En | MEDLINE | ID: mdl-35497743
In this study, we investigated the signalling pathways mediating tryptophan (Trp)-promoted ß-defensin-2 (BD-2) expression in rat intestinal mucosa. Sprague Dawley rats were administered with l-Trp and treated with rapamycin (RAPA), 1-methyltryptophan (1-MT), or para-chlorophenyl-amine (PCPA) to inhibit mammalian target of rapamycin (mTOR), indoleamine-2,3-dioxygenase (IDO), or tryptophan hydroxylase (TPH), respectively. The mRNA and protein levels of BD-2 in the jejunal and ileal mucosa of rats increased with administration of l-Trp. Intraperitoneal injection of RAPA significantly decreased the mRNA level of BD-2 and the concentrations of p-mTORC1 and BD-2 in the jejunal and ileal mucosa of rats with administration of l-Trp (P < 0.05). Oral administration of 1-MT decreased the IDO activity and the mRNA and protein levels of BD-2, and increased the concentrations of tumour necrosis factor (TNF-α), interleukin (IL)-17, and IL-22 in the jejunal and ileal mucosa of rats with administration of l-Trp (P < 0.05). Intraperitoneal injection of PCPA decreased the TPH activity and increased the mRNA and protein levels of BD-2, but did not change the concentrations of TNF-α, IL-17, or IL-22 in the jejunal and ileal mucosa of rats with administration of l-Trp. The results indicate the Trp-promoted BD-2 expression in the jejunum and ileum via the mTOR pathway and its metabolites: kynurenine banding to aryl hydrocarbon receptor in rat intestine.

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: RSC Adv Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: RSC Adv Ano de publicação: 2020 Tipo de documento: Article