Characterization on structure and bioactivities of an exopolysaccharide from Lactobacillus curvatus SJTUF 62116.
Int J Biol Macromol
; 210: 504-517, 2022 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-35508227
ABSTRACT
This study aimed to investigate the chemical structure, physicochemical properties, antioxidant capacity, antibacterial ability and anti-biofilm formation activity of an exopolysaccharide (EPS) produced by Lactobacillus curvatus SJTUF 62116 from the fish Gymnocypris przewalskii. The purified EPS, denoted as EPS-1, was mainly composed of glucose and mannose at a relative molar ratio of 11.05 with molecular weight of 31.9 kDa. The chemical structure of EPS-1 was consisted of â2)-α-D-Manp-(1â, â4)-α-D-Manp-(1â, â3,6)-α-D-Manp-(1â, T-ß-D-Glcp-(1â, â6)-ß-D-Glcp-(1â, and â3)-ß-D-Glcp-(1â glycosidic bonds. A sheet-like structure of dried EPS-1 was determined by scanning electron microscope (SEM), whilst a peak-shaped structure of EPS-1 was observed by atomic force microscope (AFM). The degradation temperature of EPS-1 was determined as 300.21 °C using thermogravimetric analysis (TGA). Moreover, the antioxidant capacity of EPS-1 at a concentration of 5.0 mg/mL against DPPH and ABTS was 84.50% and 92.53%, respectively. Furthermore, EPS-1 exhibited acceptable bacteriostatic efficacy against S. Enteritidis, E. coli, and S.aureus with significant inhibition of S. Enteritidis biofilm formation.
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Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos Bacterianos
/
Antioxidantes
Limite:
Animals
Idioma:
En
Revista:
Int J Biol Macromol
Ano de publicação:
2022
Tipo de documento:
Article