Investigation of diclofenac release and dynamic structural behavior of non-lamellar liquid crystal formulations during in situ formation by UV-Vis imaging and SAXS.
Int J Pharm
; 623: 121880, 2022 Jul 25.
Article
em En
| MEDLINE
| ID: mdl-35661744
ABSTRACT
In situ formation of high viscous inverse lyotropic non-lamellar liquid crystalline phases is a promising approach for sustained drug delivery in the joint. The in situ forming process on exposure of two diclofenac-loaded preformulations to aqueous media was characterized with respect to depot size and shape, initial release and structural transitions using UV-Vis imaging and spatially and time-resolved synchrotron small-angle X-ray scattering (SAXS). The preformulations consisted of 10 % (w/w) ethanol, 10 % (w/w) water and a binary lipid mixture of glycerol monooleate (GMO)1,2-dioleoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DOPG) or GMOmedium chain triglycerides (MCT). Upon injection of preformulations into an employed injection-cell containing excess of bio-relevant medium, rapid generation of liquid crystalline depots was observed. UV-Vis images and constructed 2D SAXS maps of the injection-cell showed depots with different shapes and sizes, and features with high nanostructural heterogeneity. More extensive swelling of the GMODOPG-based preformulation was observed compared to the GMOMCT-based preformulation. The UV image analysis found that a higher amount of diclofenac was released in the image area after 20 h from the GMOMCT depot compared to the GMODOPG depot. The injection-cell setup employing UV-Vis imaging and synchrotron SAXS constitutes an attractive approach for evaluating the in situ forming processes of liquid crystalline depots.
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01-internacional
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MEDLINE
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Cristais Líquidos
Idioma:
En
Revista:
Int J Pharm
Ano de publicação:
2022
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Article