Metal-Organic Enzyme Nanogels as Nanointegrated Self-Reporting Chemobiosensors.
ACS Appl Mater Interfaces
; 14(24): 27589-27598, 2022 Jun 22.
Article
em En
| MEDLINE
| ID: mdl-35673709
ABSTRACT
A fluorometric glucose biosensor based on fine-tuned chemoenzymatic nanohybrids is herein proposed. The successful integration of an engineered glucose oxidase enzyme and an optically responsive polymeric nanogel in a single entity has led to the fabrication of a highly efficient glucose chemobiosensor. The optical responsiveness has been achieved by the loading of preactivated polymeric hydrogel with fluorescent lanthanide, i.e., cerium (III), cations. A comprehensive investigation of the responsiveness of the biomaterial revealed the interplay between the oxidation state of the cerium lanthanide and the fluorescence emission of the polymer. Finally, a full structural, chemical, and biochemical characterization of the reported system supports the chemobiosensors as robust, specific, and sensitive materials that could be utilized to faithfully quantify the amount of glucose in tear fluids.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cério
/
Elementos da Série dos Lantanídeos
Idioma:
En
Revista:
ACS Appl Mater Interfaces
Ano de publicação:
2022
Tipo de documento:
Article