Proximity Ligation Assay for Detection of R-Loop Complexes upon DNA Damage.

Alagia, Adele; Ketley, Ruth F; Gullerova, Monika

Alagia, Adele; Ketley, Ruth F; Gullerova, Monika.

Afiliação

Alagia A; Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
Ketley RF; Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
Gullerova M; Sir William Dunn School of Pathology, University of Oxford, Oxford, UK. monika.gullerova@path.ox.ac.uk.

Methods Mol Biol ; 2528: 289-303, 2022.

Article em En | MEDLINE | ID: mdl-35704199

ABSTRACT

ABSTRACT

In situ Proximity Ligation Assay (PLA ) can be used to detect the close proximity (less than ~40 nm) of two biological molecules of interest in cells. Here we report the application of this method for the specific detection of R-loop interacting proteins and RNA modifications in close proximity to R-loops in non-damage and ionizing radiation (IR) induced DNA damage conditions.

Assuntos

Dano ao DNA; Estruturas R-Loop; Proteínas; Radiação Ionizante

Palavras-chave

DNA Damage; Double Strand breaks; Proximity Ligation Assay; R-loops; RNA modification; RNA:DNA hybrids

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dano ao DNA / Estruturas R-Loop Tipo de estudo: Diagnostic_studies Idioma: En Revista: Methods Mol Biol Ano de publicação: 2022 Tipo de documento: Article

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