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Gene expression of sex steroid metabolizing enzymes and receptors in the skeletal muscle of migrant and resident subspecies of white-crowned sparrow (Zonotrichia leucophrys).
Krause, Jesse S; Watkins, Trevor; Reid, Angus M A; Cheah, Jeffrey C; Pérez, Jonathan H; Bishop, Valerie R; Ramenofsky, Marilyn; Wingfield, John C; Meddle, Simone L.
Afiliação
  • Krause JS; Department of Biology, University of Nevada Reno, 1664 N. Virginia Street, Sarah Fleischmann 109, Reno, NV, 89557, USA. jskrause@unr.edu.
  • Watkins T; Department of Neurobiology Physiology Behavior, University of California, Davis, CA, 95616, USA. jskrause@unr.edu.
  • Reid AMA; Department of Biology, University of Nevada Reno, 1664 N. Virginia Street, Sarah Fleischmann 109, Reno, NV, 89557, USA.
  • Cheah JC; Institute of Genetics and Molecular Medicine, University of Edinburgh, Crewe Road South, Edinburgh, EH4 2XU, Scotland, UK.
  • Pérez JH; The Roslin Institute, University of Edinburgh, Midlothian, EH25 9RG, Scotland, UK.
  • Bishop VR; Department of Neurobiology Physiology Behavior, University of California, Davis, CA, 95616, USA.
  • Ramenofsky M; Department of Biology, University of South Alabama, Mobile, AL, 36688, USA.
  • Wingfield JC; The Roslin Institute, University of Edinburgh, Midlothian, EH25 9RG, Scotland, UK.
  • Meddle SL; Department of Neurobiology Physiology Behavior, University of California, Davis, CA, 95616, USA.
Oecologia ; 199(3): 549-562, 2022 Jul.
Article em En | MEDLINE | ID: mdl-35732927
ABSTRACT
Circulating sex steroid concentrations vary dramatically across the year in seasonally breeding animals. The ability of circulating sex steroids to effect muscle function can be modulated by changes in intracellular expression of steroid metabolizing enzymes (e.g., 5α-reductase type 2 and aromatase) and receptors. Together, these combined changes in plasma hormones, metabolizing enzymes and receptors allow for seasonally appropriate changes in skeletal muscle function. We tested the hypothesis that gene expression of sex steroid metabolizing enzymes and receptors would vary seasonally in skeletal muscle and these changes would differ between a migrant and resident life history strategy. We quantified annual changes in plasma testosterone and gene expression in pectoralis and gastrocnemius skeletal muscles using quantitative polymerase chain reaction (qPCR) in free-living migrant (Zonotrichia leucophrys gambelii) and resident (Z. l. nuttalli) subspecies of white-crowned sparrow during breeding, pre-basic molt, and wintering life history stages. Pectoralis muscle profile was largest in migrants during breeding, while residents maintained large muscle profiles year-round. Circulating testosterone peaked during breeding in both subspecies. Pectoralis muscle androgen receptor mRNA expression was lower in females of both subspecies during breeding. Estrogen receptor-α expression was higher in the pectoralis muscle, but not gastrocnemius, of residents throughout the annual cycle when compared to migrants. Pectoralis aromatase expression was higher in resident males compared to migrant males. No differences were observed for 5α-reductase 2. Between these two subspecies, patterns of plasma testosterone and androgen receptors appear to be conserved, however estrogen receptor gene expression appears to have diverged.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pardais Limite: Animals Idioma: En Revista: Oecologia Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pardais Limite: Animals Idioma: En Revista: Oecologia Ano de publicação: 2022 Tipo de documento: Article