[Attempt to adapt measurement of serum iron levels to the Centrifichem with a direct method using bathophenanthroline sulfonate-guanidine. Value and limits]. / Essai d'adaptation du dosage du fer sérique sur Centrifichem par une méthode directe à la bathophénanthroline sulfonée-guanidine. Intérêt et limites.

ABSTRACT

The authors have studied a direct automated colorimetric determination of iron serum on Centrifichem. Accuracy and linearity are the same than these obtained by the SFBC recommended method. Precision is poor the coefficient of variation is about 15% for low values. No alteration in the absorbance is recognized by bilirubin, even in the serum which contains 342 mumol.l-1 of bilirubin. The value of serum iron increases significantly as hemoglobin and copper are added to the serum.