Characterization of YABBY genes in Dendrobium officinale reveals their potential roles in flower development.

ABSTRACT

These YABBY genes are transcription factors (TFs) that play crucial roles in various developmental processes in plants. There is no comprehensive characterization of YABBY genes in a valuable Chinese orchid herb, Dendrobium officinale. In this study, a total of nine YABBY genes were identified in the D. officinale genome. These YABBY genes were divided into four subfamilies CRC/DL, FIL, INO, and YAB2. Expression pattern analyses showed that eight of the YABBY genes were strongly expressed in reproductive organs (flower buds) but weakly expressed in vegetative organs (roots, leaves, and stems). DoYAB1, DoYAB5, DoDL1, and DoDL3 were abundant in the small flower bud stage, while DoDL2 showed no changes throughout flower development. In addition, DoDL1-3 genes were strongly expressed in the column, tenfold more than in sepals, petals, and the lip. DoYAB1 from the FIL subfamily, DoYAB2 from the YAB2 subfamily, DoYAB3 from the INO subfamily, and DoDL2 and DoDL3 from the CRC/DL subfamily were selected for further analyses. Subcellular localization analysis showed that DoYAB1-3, DoDL2, and DoDL3 were localized in the nucleus. DoYAB2 and DoYAB3 interacted strongly with DoWOX2 and DoWOX4, while DoYAB1 showed a weak interaction with DoWOX4. These results reveal a regulatory network involving YABBY and WOX proteins in D. officinale. Our data provide clues to understanding the role of YABBY genes in the regulation of flower development in this orchid and shed additional light on the function of YABBY genes in plants.