Low Input Genome-Wide DNA Methylation Analysis with Minimal Library Amplification.
Methods Mol Biol
; 2509: 233-250, 2022.
Article
em En
| MEDLINE
| ID: mdl-35796967
ABSTRACT
Whole genome bisulfite sequencing (WGBS) is a high-throughput DNA sequencing-based technique that is used to determine genome-wide DNA methylation patterns at base resolution. Library construction by post-bisulfite adaptor tagging (PBAT ) extends the application of WGBS to several hundred cells and minimizes the required number of library amplification cycles. We herein describe a PBAT protocol to prepare WGBS libraries from 200 cells and introduce the outline of a downstream bioinformatic analysis. The prepared library can typically generate 800 million sequencing reads, which is sufficient to cover the human and mouse genomes approximately 15 times, using the Illumina NovaSeq 6000 sequencing system.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sulfitos
/
Metilação de DNA
Limite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2022
Tipo de documento:
Article