A simple and rapid mix-and-read assay for sensitive detection of O6-methylguanine DNA methyltransferase.
Chem Commun (Camb)
; 58(62): 8662-8665, 2022 Aug 02.
Article
em En
| MEDLINE
| ID: mdl-35822530
We have developed a simple and rapid mix-and-read assay for the sensitive detection of O6-methylguanine DNA methyltransferase (MGMT) activity based on exonuclease III-assisted signal amplification under completely isothermal conditions (37 °C). This method is very simple and rapid (60 min) with ultrahigh sensitivity and good specificity, and it can detect MGMT activity at the single-cell level. Moreover, this method can be applied for the screening of MGMT inhibitors and the discrimination of MGMT in different cancer cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
O(6)-Metilguanina-DNA Metiltransferase
/
Guanina
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Chem Commun (Camb)
Ano de publicação:
2022
Tipo de documento:
Article