Effects of a polysaccharide extract from Amomum villosum Lour. on gastric mucosal injury and its potential underlying mechanism.
Carbohydr Polym
; 294: 119822, 2022 Oct 15.
Article
em En
| MEDLINE
| ID: mdl-35868771
ABSTRACT
AVLP-2, a novel acidic polysaccharide, was isolated and purified from Amomum villosum Lour. The structural characteristics of the polysaccharides were characterized using monosaccharide composition, methylation, FT-IR, and NMR techniques. Results showed that AVLP-2 comprises galactose and glucose monomers, has a molecular weight of 10.488 kDa, and has backbone structures â4)-α-d-GalAp-(1â3,4)-α-d-GalAp-(1â, â4)-α-d-GalAp-(1â6)-α-d-Galp-(1â, and nâ6)-α-d-Galp-(1â4)-ß-d-Glcp-(1â, with α-d-Galp-(1â branches. Furthermore, AVLP-2 had a significant protective effect against oxidative stress in alcohol-induced injury and LPS inflammation models GES-1 cells by regulating the levels of ROS and inflammatory factors. In the animal experiments, AVLP-2 improved the oxidative stress status of the gastric mucosa by increasing SOD activity and GSH levels and inhibiting the excessive generation of malondialdehyde in tissues. The levels of MPO, IL-1ß, IL-10, and NF-κB p65 were downregulated, while that of TNF-α was upregulated by AVLP-2 treatment, thereby reducing the alcohol-induced inflammation.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Amomum
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Carbohydr Polym
Ano de publicação:
2022
Tipo de documento:
Article