Dual-Ligand Near-Infrared Luminescent Lanthanide-Based Metal-Organic Framework Coupled with In Vivo Microdialysis for Highly Sensitive Ratiometric Detection of Zn2+ in a Mouse Model of Alzheimer's Disease.
Anal Chem
; 94(34): 11940-11948, 2022 08 30.
Article
em En
| MEDLINE
| ID: mdl-35981232
ABSTRACT
Zinc, which is the second most abundant trace element in the human central nervous system, is closely associated with Alzheimer's disease (AD). However, attempts to develop highly sensitive and selective sensing systems for Zn2+ in the brain have not been successful. Here, we used a one-step solvothermal method to design and prepare a metal-organic framework (MOF) containing the dual ligands, terephthalic acid (H2BDC) and 2,2'6',2â³-terpyridine (TPY), with Eu3+ as a metal node. This MOF is denoted as Eu-MOF/BDC-TPY. Adjustment of the size and morphology of Eu-MOF/BDC-TPY allowed the dual ligands to produce multiple luminescence peaks, which could be interpreted via ratiometric fluorescence to detect Zn2+ using the ratio of Eu3+-based emission, as the internal reference, and ligand-based emission, as the indicator. Thus, Eu-MOF/BDC-TPY not only displayed higher selectivity than other metal cations but also offered a highly accurate, sensitive, wide linear, color change-based technique for detecting Zn2+ at concentrations ranging from 1 nM to 2 µM, with a low limit of detection (0.08 nM). Moreover, Eu-MOF/BDC-TPY maintained structural stability and displayed a fluorescence intensity of at least 95.4% following storage in water for 6 months. More importantly, Eu-MOF/BDC-TPY sensed the presence of Zn2+ markedly rapidly (within 5 s), which was very useful in practical application. Furthermore, the results of our ratiometric luminescent method-based analysis of Zn2+ in AD mouse brains were consistent with those obtained using inductively coupled plasma mass spectrometry.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Elementos da Série dos Lantanídeos
/
Doença de Alzheimer
/
Estruturas Metalorgânicas
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
2022
Tipo de documento:
Article