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Hydrogel Injection Molding to Generate Complex Cell Encapsulation Geometries.
Emerson, Amy E; McCall, Alec B; Brady, Sarah R; Slaby, Emily M; Weaver, Jessica D.
Afiliação
  • Emerson AE; School of Biological and Health Systems Engineering, Arizona State University, 550 East Orange Street, Tempe, Arizona 85281, United States.
  • McCall AB; School of Biological and Health Systems Engineering, Arizona State University, 550 East Orange Street, Tempe, Arizona 85281, United States.
  • Brady SR; School of Biological and Health Systems Engineering, Arizona State University, 550 East Orange Street, Tempe, Arizona 85281, United States.
  • Slaby EM; School of Biological and Health Systems Engineering, Arizona State University, 550 East Orange Street, Tempe, Arizona 85281, United States.
  • Weaver JD; School of Biological and Health Systems Engineering, Arizona State University, 550 East Orange Street, Tempe, Arizona 85281, United States.
ACS Biomater Sci Eng ; 8(9): 4002-4013, 2022 09 12.
Article em En | MEDLINE | ID: mdl-36044604
Biofabrication methods capable of generating complex, three-dimensional, cell-laden hydrogel geometries are often challenging technologies to implement in the clinic and scaled manufacturing processes. Hydrogel injection molding capitalizes on the reproducibility, efficiency, and scalability of the injection molding process, and we adapt this technique to biofabrication using a library of natural and synthetic hydrogels with varied crosslinking chemistries and kinetics. We use computational modeling to evaluate hydrogel library fluid dynamics within the injection molds in order to predict molding feasibility and cytocompatibility. We evaluate the reproducibility of hydrogel construct molding and extraction and establish criteria for the selection of hydrogels suitable for injection molding. We demonstrate that hydrogel injection molding is capable of generating complex three-dimensional cell-laden construct geometries using diverse hydrogel materials and that this platform is compatible with primary human islet encapsulation. These results highlight the versatility and feasibility of hydrogel injection molding as a biofabrication technique with potential applications in the clinic and biomanufacturing.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hidrogéis / Encapsulamento de Células Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: ACS Biomater Sci Eng Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hidrogéis / Encapsulamento de Células Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: ACS Biomater Sci Eng Ano de publicação: 2022 Tipo de documento: Article