DNA-PAINT MINFLUX nanoscopy.
Nat Methods
; 19(9): 1072-1075, 2022 09.
Article
em En
| MEDLINE
| ID: mdl-36050490
ABSTRACT
MINimal fluorescence photon FLUXes (MINFLUX) nanoscopy, providing photon-efficient fluorophore localizations, has brought about three-dimensional resolution at nanometer scales. However, by using an intrinsic on-off switching process for single fluorophore separation, initial MINFLUX implementations have been limited to two color channels. Here we show that MINFLUX can be effectively combined with sequentially multiplexed DNA-based labeling (DNA-PAINT), expanding MINFLUX nanoscopy to multiple molecular targets. Our method is exemplified with three-color recordings of mitochondria in human cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Corantes Fluorescentes
Limite:
Humans
Idioma:
En
Revista:
Nat Methods
Ano de publicação:
2022
Tipo de documento:
Article