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Cone-setting in spruce is regulated by conserved elements of the age-dependent flowering pathway.
Akhter, Shirin; Westrin, Karl Johan; Zivi, Nathan; Nordal, Veronika; Kretzschmar, Warren W; Delhomme, Nicolas; Street, Nathaniel R; Nilsson, Ove; Emanuelsson, Olof; Sundström, Jens F.
Afiliação
  • Akhter S; Department of Plant Biology, Linnean Center for Plant Biology, Uppsala BioCentre, Swedish University of Agricultural Sciences (SLU), SE-750 07, Uppsala, Sweden.
  • Westrin KJ; Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, SE-171 65, Solna, Sweden.
  • Zivi N; Department of Plant Biology, Linnean Center for Plant Biology, Uppsala BioCentre, Swedish University of Agricultural Sciences (SLU), SE-750 07, Uppsala, Sweden.
  • Nordal V; Skogforsk, Uppsala Science Park, Uppsala, SE-751 83, Sweden.
  • Kretzschmar WW; Department of Plant Biology, Linnean Center for Plant Biology, Uppsala BioCentre, Swedish University of Agricultural Sciences (SLU), SE-750 07, Uppsala, Sweden.
  • Delhomme N; Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, SE-171 65, Solna, Sweden.
  • Street NR; Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences (SLU), SE-901 83, Umeå, Sweden.
  • Nilsson O; Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, SE-901 87, Umeå, Sweden.
  • Emanuelsson O; Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences (SLU), SE-901 83, Umeå, Sweden.
  • Sundström JF; Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, SE-171 65, Solna, Sweden.
New Phytol ; 236(5): 1951-1963, 2022 12.
Article em En | MEDLINE | ID: mdl-36076311
Reproductive phase change is well characterized in angiosperm model species, but less studied in gymnosperms. We utilize the early cone-setting acrocona mutant to study reproductive phase change in the conifer Picea abies (Norway spruce), a gymnosperm. The acrocona mutant frequently initiates cone-like structures, called transition shoots, in positions where wild-type P. abies always produces vegetative shoots. We collect acrocona and wild-type samples, and RNA-sequence their messenger RNA (mRNA) and microRNA (miRNA) fractions. We establish gene expression patterns and then use allele-specific transcript assembly to identify mutations in acrocona. We genotype a segregating population of inbred acrocona trees. A member of the SQUAMOSA BINDING PROTEIN-LIKE (SPL) gene family, PaSPL1, is active in reproductive meristems, whereas two putative negative regulators of PaSPL1, miRNA156 and the conifer specific miRNA529, are upregulated in vegetative and transition shoot meristems. We identify a mutation in a putative miRNA156/529 binding site of the acrocona PaSPL1 allele and show that the mutation renders the acrocona allele tolerant to these miRNAs. We show co-segregation between the early cone-setting phenotype and trees homozygous for the acrocona mutation. In conclusion, we demonstrate evolutionary conservation of the age-dependent flowering pathway and involvement of this pathway in regulating reproductive phase change in the conifer P. abies.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Picea / Traqueófitas Idioma: En Revista: New Phytol Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Picea / Traqueófitas Idioma: En Revista: New Phytol Ano de publicação: 2022 Tipo de documento: Article