Determination of the serological sex-specific (Sxs) antigen ("H-Y antigen") in birds and mammals using high-titer antisera and a sensitive urease ELISA.
Hum Genet
; 76(4): 352-6, 1987 Aug.
Article
em En
| MEDLINE
| ID: mdl-3610155
ABSTRACT
A rapid, sensitive, and reproducible enzyme-linked immunosorbent assay (ELISA), for the detection of the serological sex-specific (Sxs) antigen (formerly termed H-Y antigen; see Introduction), is described. This assay uses bovine testes extract as the solid phase antigen, and high-titer anti-Sxs antisera and a urease-conjugated anti rat-IgG as the first and second antibody respectively. The urea containing substrate causes a pH shift in a positive reaction, which in turn is visualized by the use of bromocresol purple as a pH indicator. The method, and some representative applications of it, are described in detail.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Galinhas
/
Antígeno H-Y
/
Mamíferos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Hum Genet
Ano de publicação:
1987
Tipo de documento:
Article