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Impact of Freeze-Drying on Cord Blood (CB), Serum (S), and Platelet-Rich Plasma (CB-PRP) Preparations on Growth Factor Content and In Vitro Cell Wound Healing.
Valente, Sabrina; Ciavarella, Carmen; Astolfi, Gloria; Bergantin, Elisa; Curti, Nico; Buzzi, Marina; Fontana, Luigi; Versura, Piera.
Afiliação
  • Valente S; DIMES, Alma Mater Studiorum University of Bologna, 40138 Bologna, Italy.
  • Ciavarella C; Center for Applied Biomedical Research (CRBA), Alma Mater Studiorum University of Bologna, 40138 Bologna, Italy.
  • Astolfi G; DIMES, Alma Mater Studiorum University of Bologna, 40138 Bologna, Italy.
  • Bergantin E; Center for Applied Biomedical Research (CRBA), Alma Mater Studiorum University of Bologna, 40138 Bologna, Italy.
  • Curti N; Center for Applied Biomedical Research (CRBA), Alma Mater Studiorum University of Bologna, 40138 Bologna, Italy.
  • Buzzi M; Ophthalmology Unit, DIMES, Alma Mater Studiorum University of Bologna, 40138 Bologna, Italy.
  • Fontana L; IRCCS Azienda Ospedaliero Universitaria di Bologna, 40138 Bologna, Italy.
  • Versura P; eDIMES Lab, DIMES, Alma Mater Studiorum University of Bologna, 40138 Bologna, Italy.
Int J Mol Sci ; 23(18)2022 09 14.
Article em En | MEDLINE | ID: mdl-36142617
ABSTRACT
Blood-based preparations are used in clinical practice for the treatment of several eye disorders. The aim of this study is to analyze the effect of freeze-drying blood-based preparations on the levels of growth factors and wound healing behaviors in an in vitro model. Platelet-rich plasma (PRP) and serum (S) preparations from the same Cord Blood (CB) sample, prepared in both fresh frozen (FF) and freeze-dried (FD) forms (and then reconstituted), were analyzed for EGF and BDNF content (ELISA Quantikine kit). The human MIO-M1 glial cell line (Moorfield/Institute of Ophthalmology, London, UK) was incubated with FF and FD products and evaluated for cell migration with scratch-induced wounding (IncuCyte S3 Essen BioScience), proliferation with cyclin A2 and D1 gene expression, and activation with vimentin and GFAP gene expression. The FF and FD forms showed similar concentrations of EGF and BDNF in both the S and PRP preparations. The wound healing assay showed no significant difference between the FF and FD forms for both S and PRP. Additionally, cell migration, proliferation, and activation did not appear to change in the FD forms compared to the FF ones. Our study showed that reconstituted FD products maintained the growth factor concentrations and biological properties of FF products and could be used as a functional treatment option.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plasma Rico em Plaquetas / Ciclina A2 Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Int J Mol Sci Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plasma Rico em Plaquetas / Ciclina A2 Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Int J Mol Sci Ano de publicação: 2022 Tipo de documento: Article