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Allogeneic Bone Impregnated with Biodegradable Depot Delivery Systems for the Local Treatment of Joint Replacement Infections: An In Vitro Study.
Prokes, Libor; Snejdrova, Eva; Soukup, Tomas; Malakova, Jana; Frolov, Vladislav; Loskot, Jan; Andrys, Rudolf; Kucera, Tomas.
Afiliação
  • Prokes L; Department of Orthopaedic Surgery, University Hospital Hradec Kralove, Sokolska 581, 500 05 Hradec Kralove, Czech Republic.
  • Snejdrova E; Faculty of Medicine, Charles University, Simkova 870, 500 03 Hradec Kralove, Czech Republic.
  • Soukup T; Department of Pharmaceutical Technology, Faculty of Pharmacy, Charles University, Akademika Heyrovskeho 1203, 500 05 Hradec Kralova, Czech Republic.
  • Malakova J; Department of Histology and Embryology, Faculty of Medicine, Charles University, Simkova 870, 500 03 Hradec Kralove, Czech Republic.
  • Frolov V; Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Kralove, Sokolska 581, 500 05 Hradec Kralove, Czech Republic.
  • Loskot J; Department of Pharmaceutical Technology, Faculty of Pharmacy, Charles University, Akademika Heyrovskeho 1203, 500 05 Hradec Kralova, Czech Republic.
  • Andrys R; Department of Physics, Faculty of Science, University of Hradec Kralove, Rokitanskeho 62, 500 03 Hradec Kralove, Czech Republic.
  • Kucera T; Department of Chemistry, Faculty of Science, University of Hradec Kralove, Rokitanskeho 62, 500 03 Hradec Kralove, Czech Republic.
Molecules ; 27(19)2022 Oct 01.
Article em En | MEDLINE | ID: mdl-36235024
Although progress is evident in the effective treatment of joint replacement-related infections, it still remains a serious issue in orthopedics. As an example, the local application of antibiotics-impregnated bone grafts supplies the high drug levels without systemic side effects. However, antibiotics in the powder or solution form could be a risk for local toxicity and do not allow sustained drug release. The present study evaluated the use of an antibiotic gel, a water-in-oil emulsion, and a PLGA microparticulate solid dispersion as depot delivery systems impregnating bone grafts for the treatment of joint replacement-related infections. The results of rheological and bioadhesive tests revealed the suitability of these formulations for the impregnation of bone grafts. Moreover, no negative effect on proliferation and viability of bone marrow mesenchymal stem cells was detected. An ex vivo dissolution test of vancomycin hydrochloride and gentamicin sulphate from the impregnated bone grafts showed a reduced burst and prolonged drug release. The PLGA-based formulation proved to be particularly promising, as one-day burst release drugs was only 15% followed with sustained antibiotics release with zero-order kinetics. The results of this study will be the basis for the development of a new product in the Tissue Section of the University Hospital for the treatment of bone defects and infections of joint replacements.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transplante de Células-Tronco Hematopoéticas / Artroplastia de Substituição Limite: Humans Idioma: En Revista: Molecules Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transplante de Células-Tronco Hematopoéticas / Artroplastia de Substituição Limite: Humans Idioma: En Revista: Molecules Ano de publicação: 2022 Tipo de documento: Article