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GPNMB promotes abdominal fat deposition in chickens: genetic variation, expressional profile, biological function, and transcriptional regulation.
Wang, Dandan; Teng, Menglin; Wang, Yangyang; Cao, Yuzhu; Tian, Weihua; Wang, Zhang; Guo, Yulong; Li, Hong; Li, Zhuanjian; Jiang, Ruirui; Li, Guoxi; Tian, Yadong; Liu, Xiaojun.
Afiliação
  • Wang D; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
  • Teng M; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
  • Wang Y; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
  • Cao Y; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
  • Tian W; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
  • Wang Z; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
  • Guo Y; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
  • Li H; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; Henan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Zhengzhou 450046, China; International Joint Research Laboratory for Poultry Breeding of Henan, Zhengzhou 450046, Chin
  • Li Z; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; Henan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Zhengzhou 450046, China; International Joint Research Laboratory for Poultry Breeding of Henan, Zhengzhou 450046, Chin
  • Jiang R; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; Henan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Zhengzhou 450046, China; International Joint Research Laboratory for Poultry Breeding of Henan, Zhengzhou 450046, Chin
  • Li G; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; Henan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Zhengzhou 450046, China; International Joint Research Laboratory for Poultry Breeding of Henan, Zhengzhou 450046, Chin
  • Tian Y; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; Henan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Zhengzhou 450046, China; International Joint Research Laboratory for Poultry Breeding of Henan, Zhengzhou 450046, Chin
  • Liu X; College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China; Henan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, Zhengzhou 450046, China; International Joint Research Laboratory for Poultry Breeding of Henan, Zhengzhou 450046, Chin
Poult Sci ; 101(12): 102216, 2022 Dec.
Article em En | MEDLINE | ID: mdl-36279606
Glycoprotein nonmetastatic melanoma protein B (GPNMB) is a vital secreted factor that promotes the occurrence of obesity in mammals. However, the effects of GPNMB on abdominal fat deposition is still unknown in chickens. In this study, we looked into the genetic and expression association of GPNMB gene with abdominal fat traits in chicken, and found that a genetic variation rs31126482 in GPNMB promoter was significantly associated with abdominal fat weight (AFW, P < 0.05) and abdominal fat percentage (AFP, P < 0.01). Express profile analysis of the GPNMB indicated that the gene was mainly expressed in abdominal fat tissue, and its expression level was strongly positively correlated with AFW (R2 = 0.6356, P = 4.10E-05) and AFP (R2 = 0.6450, P = 2.90E-05). We then investigated biological function of GPNMB on adipogenesis in chicken, and found that GPNMB could inhibit abdominal preadipocyte proliferation, but promote abdominal preadipocyte differentiation and lipid deposition. Furthermore, we explored regulatory mechanism of GPNMB gene in chicken, and detected one nonclassical estrogen regulatory element (AP1) and one peroxisome proliferator-activated receptor α (PPARα) responsive element in the 2 kb promoter region of GPNMB gene, and demonstrated that estrogen could up-regulate GPNMB mRNA expression in adipose tissue and primary abdominal preadipocytes, while PPARα could down-regulate GPNMB expression in primary preadipocytes. Taken together, this study brings new insights into understanding the function and transcriptional control of GPNMB gene, and provides genetic markers for breeding selection to improve abdominal fat traits in chicken.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Galinhas / PPAR alfa Limite: Animals Idioma: En Revista: Poult Sci Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Galinhas / PPAR alfa Limite: Animals Idioma: En Revista: Poult Sci Ano de publicação: 2022 Tipo de documento: Article