Highly sensitive benzothiazole-based chemosensors for detection and bioimaging of peroxynitrite in living cells.
RSC Adv
; 12(43): 27933-27939, 2022 Sep 28.
Article
em En
| MEDLINE
| ID: mdl-36320233
ABSTRACT
It is well accepted that peroxynitrite (ONOO-) plays a crucial role in various physiological and pathological processes. Thus, the detection and imaging of ONOO- in vitro and in vivo with high selectivity and sensitivity is of great significance. Here we report two simple benzothiazole-based fluorescent chemosensors, BS1 and BS2. Under physiological pH, both probes could quickly sense ONOO- with a remarkable "turn-on" fluorescence signal at 430 nm. The limit of detection (LOD) of BS1 and BS2 toward ONOO- was 12.8 nM and 25.2 nM, respectively, much lower than the reported values. Experimental results indicated that BS1 with a diphenyl phosphonate unit presented higher selectivity for ONOO- than BS2. Furthermore, based on the advantages of lower cytotoxicity and pH-stabilities of BS1, probe BS1 was successfully employed to detect and image ONOO- in HepG2 cells. More importantly, we used BS1 to successfully showcase drug-induced hepatotoxicity via imaging ONOO- upregulated by acetaminophen (APAP), and also evaluated the remediation effect of GSH. All the results illustrated that the fluorescent probe BS1 has great potential for the detection of ONOO- and to further uncover the roles of ONOO- during the drug-induced liver injury (DILI) process.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
RSC Adv
Ano de publicação:
2022
Tipo de documento:
Article