A CRISPR endonuclease gene drive reveals distinct mechanisms of inheritance bias.
Nat Commun
; 13(1): 7145, 2022 11 21.
Article
em En
| MEDLINE
| ID: mdl-36414618
ABSTRACT
CRISPR/Cas gene drives can bias transgene inheritance through different mechanisms. Homing drives are designed to replace a wild-type allele with a copy of a drive element on the homologous chromosome. In Aedes aegypti, the sex-determining locus is closely linked to the white gene, which was previously used as a target for a homing drive element (wGDe). Here, through an analysis using this linkage we show that in males inheritance bias of wGDe did not occur by homing, rather through increased propagation of the donor drive element. We test the same wGDe drive element with transgenes expressing Cas9 with germline regulatory elements sds3, bgcn, and nup50. We only find inheritance bias through homing, even with the identical nup50-Cas9 transgene. We propose that DNA repair outcomes may be more context dependent than anticipated and that other previously reported homing drives may, in fact, bias their inheritance through other mechanisms.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Aedes
/
Tecnologia de Impulso Genético
Limite:
Animals
Idioma:
En
Revista:
Nat Commun
Ano de publicação:
2022
Tipo de documento:
Article