An alternative σ factor σL sl regulates lincomycin production in Streptomyces lincolnensis.
J Basic Microbiol
; 63(2): 190-199, 2023 Feb.
Article
em En
| MEDLINE
| ID: mdl-36453540
ABSTRACT
Lincomycin produced by Streptomyces lincolnensis is a critical antibacterial antibiotic in the clinical. To further understand the regulatory mechanism of lincomycin biosynthesis, we identified an alternative σ factor, σL sl , in Streptomyces lincolnensis NRRL 2936. Deletion of sigLsl resulted in an increase in cell growth but a decrease in lincomycin production. σL sl boosted lincomycin biosynthesis by directly stimulating the transcription of four genes (lmbD, lmbV, lmrC, and lmbU) within the lincomycin biosynthetic lmb gene cluster. Besides, σL sl participated in lincomycin biosynthesis by directly stimulating the transcription of mshC, a gene responsible for MSH synthesis. In conclusion, our findings demonstrated that σL sl plays a direct regulatory role in lincomycin biosynthesis. This study extends the understanding of molecular mechanisms of lincomycin biosynthetic regulation.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fator sigma
/
Lincomicina
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
J Basic Microbiol
Ano de publicação:
2023
Tipo de documento:
Article