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An ER-targeted "reserve-release" fluorogen for topological quantification of reticulophagy.
Fang, Hongbao; Hu, Lianting; Chen, Qixin; Geng, Shanshan; Qiu, Kangqiang; Wang, Chengjun; Hao, Mingang; Tian, Zhiqi; Chen, Huimin; Liu, Lei; Guan, Jun-Lin; Chen, Yuncong; Dong, Lei; Guo, Zijian; He, Weijiang; Diao, Jiajie.
Afiliação
  • Fang H; State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China; Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA; Schoo
  • Hu L; Medical Big Data Center, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China; Guangdong Cardiovascular Institute, Guangzhou, 510080, China; School of Information Management, Wuhan University, Wuhan 430072, China.
  • Chen Q; Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA; Institute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250062, China.
  • Geng S; State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
  • Qiu K; Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA.
  • Wang C; Sinopec Shengli Petroleum Engineering Limited Company, Dongying, 257000, China.
  • Hao M; Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA.
  • Tian Z; Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA.
  • Chen H; Institute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250062, China.
  • Liu L; Institute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250062, China.
  • Guan JL; Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA.
  • Chen Y; State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China. Electronic address: chenyc@nju.edu.cn.
  • Dong L; School of Life Sciences, Nanjing University, Nanjing 210023, China.
  • Guo Z; State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China. Electronic address: zguo@nju.edu.cn.
  • He W; State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China. Electronic address: heweij69@nju.edu.cn.
  • Diao J; Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA. Electronic address: jiajie.diao@uc.edu.
Biomaterials ; 292: 121929, 2023 01.
Article em En | MEDLINE | ID: mdl-36455487
The endoplasmic reticulum's (ER) dynamic nature, essential for maintaining cellular homeostasis, can be influenced by stress-induced damage, which can be assessed by examining the morphology of ER dynamics and, more locally, ER properties such as hydrophobicity, viscosity, and polarity. Although numerous ER-specific chemical probes have been developed to monitor the ER's physical and chemical parameters, the quantitative detection and super-resolution imaging of its local hydrophobicity have yet to be explored. Here, we describe a photostable ER-targeted probe with high signal-to-noise ratio for super-resolution imaging that can specifically respond to changes in ER hydrophobicity under stress based on a "reserve-release" mechanism. The probe shows an excellent ability to target ER over commercial ER dyes and can be used to track local changes of hydrophobicity by fluorescence intensity and morphology during the selective autophagy of ER (i.e., reticulophagy). By correlating the level and location of ER damage with the distribution of fluorescence intensity, we were able to assess reticulophagy at the subcellular level. Beyond that, we developed a topological analytical tool adaptable to any ER probe for detecting structural changes in ER and thus quantitatively identifying reticulophagy. The algorithm-assisted tool can also be adapted to a wide range of molecular probes and organelles. Altogether, the new probe and analytical strategy described here show promise for the quantitative detection and analysis of subtle ER damage and stress.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Autofagia / Retículo Endoplasmático Idioma: En Revista: Biomaterials Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Autofagia / Retículo Endoplasmático Idioma: En Revista: Biomaterials Ano de publicação: 2023 Tipo de documento: Article