Dual-Channel Fluorescent Probe for Detecting Viscosity and ONOO- without Signal Crosstalk in Nonalcoholic Fatty Liver.
Anal Chem
; 94(50): 17439-17447, 2022 12 20.
Article
em En
| MEDLINE
| ID: mdl-36475623
ABSTRACT
Nonalcoholic fatty liver disease (NAFLD) is a global health issue. Peroxynitrite and liver viscosity have recently been found to be potential biomarkers of NAFLD. Therefore, it is of great significance to develop dual-response fluorescent probes for simultaneous detecting peroxynitrite and viscosity. We report herein a new probe (CQ) that can simultaneously detect peroxynitrite and viscosity at two independent fluorescent channels without signal crosstalk. CQ shows high selectivity, rapid response, good water solubility, low cytotoxicity, and mitochondrial localization properties. In particular, CQ responds sensitively to viscosity and peroxynitrite with off-on fluorescence changes at 710 and 505 nm, respectively. The wavelength gap between these two channels is more than 200 nm, ensuring that there is no signal crosstalk during detection. With this property, the probe was applied to simultaneously detect mitochondrial viscosity and peroxynitrite and image the changes of liver viscosity and peroxynitrite concentration during the pathogenesis of NAFLD. All results show that the CQ probe is a powerful tool for simultaneous detection of viscosity and peroxynitrite and provides a potential new diagnostic method for NAFLD.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
1_ASSA2030
/
2_ODS3
Base de dados:
MEDLINE
Assunto principal:
Hepatopatia Gordurosa não Alcoólica
/
Corantes Fluorescentes
Limite:
Humans
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
2022
Tipo de documento:
Article