Genome Editing Using Cas9 Ribonucleoprotein Is Effective for Introducing <i>PDGFRA</i> Variant in Cultured Human Glioblastoma Cell Lines.

Hamada, Taiji; Yokoyama, Seiya; Akahane, Toshiaki; Matsuo, Kei; Tanimoto, Akihide

Genome Editing Using Cas9 Ribonucleoprotein Is Effective for Introducing PDGFRA Variant in Cultured Human Glioblastoma Cell Lines.

Hamada, Taiji; Yokoyama, Seiya; Akahane, Toshiaki; Matsuo, Kei; Tanimoto, Akihide.

Afiliação

Hamada T; Department of Pathology, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544, Japan.
Yokoyama S; Department of Pathology, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544, Japan.
Akahane T; Department of Pathology, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544, Japan.
Matsuo K; Department of Pathology, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544, Japan.
Tanimoto A; Department of Pathology, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8544, Japan.

Int J Mol Sci ; 24(1)2022 Dec 28.

Article em En | MEDLINE | ID: mdl-36613947

Assuntos

Edição de Genes; Glioblastoma; Humanos; Edição de Genes/métodos; Sistemas CRISPR-Cas/genética; Glioblastoma/genética; Ribonucleoproteínas/genética; Ribonucleoproteínas/metabolismo; Linhagem Celular; Nucleotídeos/metabolismo

Palavras-chave

Cas9 ribonucleoprotein; PDGFRA; cultured glioblastoma cell lines; single-nucleotide substitution; transfection methods

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Glioblastoma / Edição de Genes Limite: Humans Idioma: En Revista: Int J Mol Sci Ano de publicação: 2022 Tipo de documento: Article

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