Jaw1/LRMP is associated with the maintenance of Golgi ribbon structure.

Okumura, Wataru; Tadahira, Kazuko; Kozono, Takuma; Tamura-Nakano, Miwa; Sato, Hiroyuki; Matsui, Hitomi; Dohi, Taeko; Rohrer, Jack; Tonozuka, Takashi; Nishikawa, Atsushi

Okumura, Wataru; Tadahira, Kazuko; Kozono, Takuma; Tamura-Nakano, Miwa; Sato, Hiroyuki; Matsui, Hitomi; Dohi, Taeko; Rohrer, Jack; Tonozuka, Takashi; Nishikawa, Atsushi.

Afiliação

Okumura W; Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
Tadahira K; Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
Kozono T; Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
Tamura-Nakano M; Communal Laboratory, Research Institute, National Center for Global Health and Medicine, Tokyo 162-8655, Japan.
Sato H; Cooperative Major in Advanced Health Science, Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
Matsui H; Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
Dohi T; Department of Gastroenterology, Research Center for Hepatitis and Immunology, Research Institute, National Center for Global Health and Medicine, Chiba 272-8516, Japan.
Rohrer J; Institute of Chemistry and Biotechnology, Zurich University of Applied Sciences, Waedenswil, CH-8820, Switzerland.
Tonozuka T; Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
Nishikawa A; Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.

J Biochem ; 173(5): 383-392, 2023 Apr 26.

Article em En | MEDLINE | ID: mdl-36689741

ABSTRACT

ABSTRACT

Jaw1/LRMP is a membrane protein that is localized to the endoplasmic reticulum and outer nuclear membrane. Previously, we revealed that Jaw1 functions to maintain nuclear shape by interacting with microtubules as a Klarsicht/ANC-1/Syne/homology (KASH) protein. The loss of several KASH proteins causes defects in the position and shape of the Golgi apparatus as well as the nucleus, but the effects of Jaw1 depletion on the Golgi apparatus were poorly understood. Here, we found that siRNA-mediated Jaw1 depletion causes Golgi fragmentation with disordered ribbon structure in the melanoma cell, accompanied by the change in the localization of the Golgi-derived microtubule network. Thus, we suggest that Jaw1 is a novel protein to maintain the Golgi ribbon structure, associated with the microtubule network.

Assuntos

Núcleo Celular; Complexo de Golgi; Membrana Nuclear; Núcleo Celular/metabolismo; Citoesqueleto/metabolismo; Complexo de Golgi/metabolismo; Microtúbulos; Membrana Nuclear/metabolismo

Palavras-chave

Golgi apparatus.Abbreviations: Ac-Tubulin, Acetylated-Tubulin; CLASPs, CLIP-associated proteins; DNase I, Deoxyribonuclease I; ER, Endoplasmic reticulum; GM130, Golgi matrix protein 130 kDa; GRASP65, Golgi reassembly-stacking protein 1; GRASP55, Golgi reassembly-stacking protein 2; HRP, Horseradish peroxidase; ITPR, Inositol 1,4,5-trisphosphate receptor; KASH, Klarsicht/ANC-1/Syne/homology; KD, knockdown; KIF20A, Kinesin-like protein KIF20A; LINC, Linker of nucleus and cytoskeletons; LRMP, Lymphoid restricted membrane protein; MTCL1, Microtubule cross-linking factor 1; NT, Non-targeting; ONM, outer nuclear membrane; siRNA, short interfering RNA; SUN, Sad-1/UNC-84; TEM, transmission electron microscopy; Jaw1/LRMP; KASH protein; LINC complex; microtubule network

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Núcleo Celular / Complexo de Golgi / Membrana Nuclear Tipo de estudo: Risk_factors_studies Idioma: En Revista: J Biochem Ano de publicação: 2023 Tipo de documento: Article

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