Distinction of Male and Female Trees of Ginkgo biloba Using LAMP.
Mol Biotechnol
; 65(10): 1693-1703, 2023 Oct.
Article
em En
| MEDLINE
| ID: mdl-36745281
ABSTRACT
Ginkgo biloba is utilized as food, medicine, wood, and street trees among other things. The objective of this study was to develop a loop-mediated isothermal amplification (LAMP) assay for gender distinction of G. biloba. Male-specific SCAR gene can be utilized to identify G. biloba gender using LAMP. The optimized LAMP conditions, temperature 60 °C, 2-mM MgSO4, and [F3/B3][FIP/BIP] primer ratio of 14 were selected as final conditions. The G. biloba SCAR LAMP displayed a sensitivity of 10 ng when amplified by concentration under the optimum conditions. Additionally, it demonstrated a particular response in male with SYBR Green I in LAMP analysis that can be a more powerful tool for field and scale-up applications. Our work represents a first attempt to identify G. biloba gender using LAMP and offers an efficient and reliable tool for roadside landscaping.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Árvores
/
Ginkgo biloba
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Mol Biotechnol
Ano de publicação:
2023
Tipo de documento:
Article