Actinotalea soli sp. nov., isolated from Kubuqi Desert soil.
Int J Syst Evol Microbiol
; 72(12)2022 Dec.
Article
em En
| MEDLINE
| ID: mdl-36748610
ABSTRACT
A Gram-stain positive, facultatively anaerobic, motile rod-shaped strain, BY-33T, was isolated from a soil sample obtained from the Kubuqi Desert, PR China. Phylogenetic analysis based on 16S rRNA gene sequence similarity revealed that strain BY-33T was most closely related to the genus Actinotalea, including Actinotalea ferrariae CF5-4T (98.2â% similarity), 'Actinotalea subterranea' HO-Ch2T (98.0â%), Actinotalea solisilvae THG-T121T (97.6â%), 'Actinotalea bogoriensis' 69B4T (97.5â%), Actinotalea fermentans MT (97.3â%) and 'Actinotalea carbonis' T26T (97.0â%). The strain grew at 0â37 °C (optimum, 28-30 °C) and pH 6.0-11.0 (optimum, pH 9.0-10.0) and with 0â8.0â% (w/v) NaCl (optimum, 3.0%) on tryptic soy agar. It had catalase activity, but no oxidase activity. The polar lipids of strain BY-33T contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and phosphatidylinositol mannosides. The major respiratory quinone of strain BY-33T was MK-10 (H4). Its major fatty acids were anteiso-C15ââ0, anteiso-C15ââ1 A and C16ââ0. The genomic DNA G+C content of strain BY-33T was 73.0âmol% based on total genome calculations. The average nucleotide identity scores between the genomic sequences of strain BY-33T and the other species of the genus Actinotalea were found to be low (ANIm <85.0â%, ANIb <77.0â% and OrthoANIu <78.0â%). Furthermore, the digital DNA-DNA hybridization and average amino acid identity values between strain BY-33T and the closely related species ranged from 20.5 to 21.0% and from 62.2 to 72.2â%, respectively. Based on the results of phylogenetic, phenotypic, genotypic and chemotaxonomic analyses, it is concluded that strain BY-33T represents a novel species within the genus Actinotalea, for which the name Actinotalea soli sp. nov. is proposed. The type strain is BY-33T (=CGMCC 1.17460T=KCTC 49362T).
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Actinomycetales
País/Região como assunto:
Asia
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Ano de publicação:
2022
Tipo de documento:
Article