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Construction and Evaluation of Zinc Finger Nucleases.
Ochiai, Hiroshi; Yamamoto, Takashi.
Afiliação
  • Ochiai H; Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Hiroshima, Japan. ochiai@hiroshima-u.ac.jp.
  • Yamamoto T; Genome Editing Innovation Center, Hiroshima University, Higashi-Hiroshima, Hiroshima, Japan. ochiai@hiroshima-u.ac.jp.
Methods Mol Biol ; 2637: 1-25, 2023.
Article em En | MEDLINE | ID: mdl-36773134
Zinc finger nucleases (ZFNs) are programmable nucleases that have contributed significantly to past genome-editing research. They are now utilized much less owing to the advent of transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeats and CRISPR-associated protein system (CRISPR-Cas). These new methods allow for easier generation of reagents that target genomic sequences of interest and are less labor-intensive than ZFNs at targeting desired sequences. However, fundamental ZFN patents have expired, enabling a wide range of their distribution for clinical and industrial applications. This article introduces a ZFN construction protocol that uses bacterial one-hybrid (B1H) selection and single-strand annealing (SSA) assay.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sistemas CRISPR-Cas / Nucleases de Dedos de Zinco Tipo de estudo: Guideline Idioma: En Revista: Methods Mol Biol Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sistemas CRISPR-Cas / Nucleases de Dedos de Zinco Tipo de estudo: Guideline Idioma: En Revista: Methods Mol Biol Ano de publicação: 2023 Tipo de documento: Article