BACTERIAL CONTAMINATION AND METHODS OF DECONTAMINATION OF BASES COMPLETE REMOVABLE PROSTHESES DURING THE APPLICATION OF ADHESIVE MATERIALS.

ABSTRACT

It turned out that the permanent microbiota of surfaces of acrylic bases of complete removable plastic dentures (CRPD) characterized by several features the presence of aerobic (7) and anaerobic (10) species. Qualitative and quantitative compositions of microbiocenosis in orthopedic treatment stages have been changed. The revealed patterns require further study in the context of the improvement of decolonization of acrylic bases in the stages of orthopedic treatment by CRPD. Deactivation of acrylic bases of CRPD significantly affects the species composition of microbiota, reduces the quantitative parameters of bacterial contamination. The purpose of our work was determination of bacterial contamination of acrylic bases of CRPD in stages of their clinical exploitation and study of changes in the quantitative composition of the acrylic bases microbiota of CRPD under different decontamination modes. Smears from the inner surface of the acrylic base were stained with Gram and microscopic and seeded on blood agar, Endo agar, Chistovich, Saburo. Species belonging to the micro-organisms were identified by the LAHEMA test system and were determined in colonies forming units (CFUs). These data indicate accumulation under the acrylic base in the course of clinical operation of certain types of microbiota, which requires improvement of the methods of their decontamination. We studied the change in the quantitative and specific composition of the microbiota (bacterial contamination) of acrylic bases CRPD under different modes ("A", "B"). Patients in group "A" used the Sideex solution - a two-component system consisting of a liquid component (glutaraldehyde solution) and a powdery activator, mixed before use to obtain a working activated solution. The liquid component is a clear, colorless solution with a specific odor, which is a 2.2-2.7% aqueous solution of glutaraldehyde, which is an active ingredient, pH=3.0 - 4.5. Powdery activator is a pale-yellow powder containing alkaline components, a corrosion inhibitor, and a dye. The activated working solution is a fluorescent green solution with a specific odor and containing 2.2-2.7% glutaraldehyde; pH=8.2-9.2. (Great Britain). Preparation of the activated solution a powder-activator is added to the container containing the liquid component (avoiding losses). Cleaning is carried out by the method of full immersion of the prosthesis in solution, with a thickness of the drug over it not less than 1 cm. The dentures are soused in solution for 15 minutes, then thorough washing in the same solution for 1-3 minutes. Sideex activated solution is used for sterilization and disinfection of metal, glass, polymeric (plastic, rubber, etc.) medical products. The dentures of patients from group "B" are decontaminated with 0.2% solution of chlorhexidine bigluconate. The dentures were placed in the solution overnight for 14 days, changing the solution every two days. The decontamination of acrylic bases of CRPD should be considered mode "B" as more effective because of the significant decrease in the species composition of the microbiota (the qualitative indicator of reduction of microbial contamination was 72.0%). It is substantiated that decontamination of acrylic bases of CRPD with mode "A" has a small effect on the species composition of the microbiota, although it significantly (p<0.05) reduces quantitative indicators of microbial contamination. Regarding "B" mode, the species composition of aerobes from 31 lgCFU/ml to 8.7 lgCFU/ml and anaerobes from 42.7 lgCFU/ml to 14.6 lgCFU/ml was considered more effective.