The use of tyrosinases in a chemoenzymatic cascade as a peptide ligation strategy.
RSC Chem Biol
; 4(2): 132-137, 2023 Feb 08.
Article
em En
| MEDLINE
| ID: mdl-36794017
ABSTRACT
Peptides play many key roles in biological systems and numerous methods have been developed to generate both natural and unnatural peptides. However, straightforward, reliable coupling methods that can be achieved under mild reactions conditions are still sought after. In this work, a new N-terminal tyrosine-containing peptide ligation method with aldehydes, utilising a Pictet-Spengler reaction is described. In a key step, tyrosinase enzymes have been used to convert l-tyrosine to l-3,4-dihydroxyphenyl alanine (l-DOPA) residues, generating suitable functionality for the Pictet-Spengler coupling. This new chemoenzymatic coupling strategy can be used for fluorescent-tagging and peptide ligation purposes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
RSC Chem Biol
Ano de publicação:
2023
Tipo de documento:
Article