A high-temperature sensitivity of Synechococcus elongatus PCC 7942 due to a tRNA-Leu mutation.
J Gen Appl Microbiol
; 69(3): 167-174, 2023 Dec 05.
Article
em En
| MEDLINE
| ID: mdl-36805585
ABSTRACT
Certain mutations of the model cyanobacterium Synechococcus elongatus PCC 7942 during laboratory storage have resulted in some divergent phenotypes. One laboratory-stored strain (H1) shows a temperature-sensitive (ts) growth phenotype at 40 °C. Here, we investigated the reason for this temperature sensitivity. Whole genome sequencing of H1 identified a single nucleotide mutation in synpcc7942_R0040 encoding tRNA-Leu(CAA). The mutation decreases the length of the tRNA-Leu t-arm from 5 to 4 base pairs, and this explains the ts phenotype. Secondary mutations suppressing the ts phenotype were identified in synpcc7942_1640, which putatively encodes a NYN domain-containing protein (nynA). The NYN domain is thought to be involved in tRNA/rRNA degradation. Thus, the structural stability of tRNA-Leu is critical for growth at 40 °C in Synechococcus elongatus PCC 7942.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA de Transferência de Leucina
/
Synechococcus
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
J Gen Appl Microbiol
Ano de publicação:
2023
Tipo de documento:
Article