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High-plex protein and whole transcriptome co-mapping at cellular resolution with spatial CITE-seq.
Liu, Yang; DiStasio, Marcello; Su, Graham; Asashima, Hiromitsu; Enninful, Archibald; Qin, Xiaoyu; Deng, Yanxiang; Nam, Jungmin; Gao, Fu; Bordignon, Pino; Cassano, Marco; Tomayko, Mary; Xu, Mina; Halene, Stephanie; Craft, Joseph E; Hafler, David; Fan, Rong.
Afiliação
  • Liu Y; Department of Biomedical Engineering, Yale University, New Haven, CT, USA.
  • DiStasio M; Yale Stem Cell Center and Yale Cancer Center, Yale School of Medicine, New Haven, CT, USA.
  • Su G; Department of Pathology, Yale School of Medicine, New Haven, CT, USA.
  • Asashima H; Department of Medicine, Yale School of Medicine, New Haven, CT, USA.
  • Enninful A; Department of Neurology, Yale School of Medicine, New Haven, CT, USA.
  • Qin X; Department of Pathology, Yale School of Medicine, New Haven, CT, USA.
  • Deng Y; Department of Medicine, Yale School of Medicine, New Haven, CT, USA.
  • Nam J; Department of Neurology, Yale School of Medicine, New Haven, CT, USA.
  • Gao F; Department of Biomedical Engineering, Yale University, New Haven, CT, USA.
  • Bordignon P; Yale Stem Cell Center and Yale Cancer Center, Yale School of Medicine, New Haven, CT, USA.
  • Cassano M; Department of Medicine, Yale School of Medicine, New Haven, CT, USA.
  • Tomayko M; Department of Neurology, Yale School of Medicine, New Haven, CT, USA.
  • Xu M; Department of Biomedical Engineering, Yale University, New Haven, CT, USA.
  • Halene S; Yale Stem Cell Center and Yale Cancer Center, Yale School of Medicine, New Haven, CT, USA.
  • Craft JE; Department of Biomedical Engineering, Yale University, New Haven, CT, USA.
  • Hafler D; Yale Stem Cell Center and Yale Cancer Center, Yale School of Medicine, New Haven, CT, USA.
  • Fan R; Department of Biomedical Engineering, Yale University, New Haven, CT, USA.
Nat Biotechnol ; 41(10): 1405-1409, 2023 Oct.
Article em En | MEDLINE | ID: mdl-36823353
ABSTRACT
In this study, we extended co-indexing of transcriptomes and epitopes (CITE) to the spatial dimension and demonstrated high-plex protein and whole transcriptome co-mapping. We profiled 189 proteins and whole transcriptome in multiple mouse tissue types with spatial CITE sequencing and then further applied the method to measure 273 proteins and transcriptome in human tissues, revealing spatially distinct germinal center reactions in tonsil and early immune activation in skin at the Coronavirus Disease 2019 mRNA vaccine injection site.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Análise de Célula Única / Transcriptoma Limite: Animals / Humans Idioma: En Revista: Nat Biotechnol Ano de publicação: 2023 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Análise de Célula Única / Transcriptoma Limite: Animals / Humans Idioma: En Revista: Nat Biotechnol Ano de publicação: 2023 Tipo de documento: Article