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Mucin Protein of Aedes aegypti Interacts with Dengue Virus 2 and Influences Viral Infection.
Yadav, Karuna; Rana, Vipin Singh; Saurav, Gunjan Kumar; Rawat, Nitish; Kumar, Ankit; Sunil, Sujatha; Singh, Om P; Rajagopal, Raman.
Afiliação
  • Yadav K; Gut Biology Laboratory, Department of Zoology, University of Delhi, New Delhi, India.
  • Rana VS; Gut Biology Laboratory, Department of Zoology, University of Delhi, New Delhi, India.
  • Anjali; Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA.
  • Saurav GK; Gut Biology Laboratory, Department of Zoology, University of Delhi, New Delhi, India.
  • Rawat N; Gut Biology Laboratory, Department of Zoology, University of Delhi, New Delhi, India.
  • Kumar A; Department of Zoology, Rajiv Gandhi University, Doimukh, Arunachal Pradesh, India.
  • Sunil S; Gut Biology Laboratory, Department of Zoology, University of Delhi, New Delhi, India.
  • Singh OP; Vector Borne Disease Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.
  • Rajagopal R; Vector Borne Disease Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India.
Microbiol Spectr ; : e0250322, 2023 Feb 27.
Article em En | MEDLINE | ID: mdl-36847498
Dengue, caused by dengue virus (DENV), is the most prevalent vector-borne viral disease, posing a serious health concern to 2.5 billion people worldwide. DENV is primarily transmitted among humans by its mosquito vector Aedes aegypti; hence, the identification of a novel dengue virus receptor in mosquitoes is critical for the development of new anti-mosquito measures. In the current study, we have identified peptides which potentially interact with the surface of the virion particles and facilitate virus infection and movement during their life cycle in the mosquito vector. To identify these candidate proteins, we performed phage-display library screening against domain III of the envelope protein (EDIII), which plays an essential role during host cell receptor binding for viral entry. The mucin protein, which shared sequence similarity with the peptide identified in the screening, was cloned, expressed, and purified for in vitro interaction studies. Using in vitro pulldown and virus overlay protein-binding assay (VOPBA), we confirmed the positive interaction of mucin with purified EDIII and whole virion particles. Finally, blocking of mucin protein with anti-mucin antibodies partially reduced DENV titers in infected mosquitos. Moreover, mucin protein was found to be localized in the midgut of Ae. aegypti. IMPORTANCE Identification of interacting protein partners of DENV in the insect vector Aedes aegypti is crucial for designing vector control-based strategies and for understanding the molecular mechanism DENV uses to modulate the host, gain entry, and survive successfully. Similar proteins can be used in generating transmission-blocking vaccines.
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Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 2_ODS3 / 3_ND Base de dados: MEDLINE Idioma: En Revista: Microbiol Spectr Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 2_ODS3 / 3_ND Base de dados: MEDLINE Idioma: En Revista: Microbiol Spectr Ano de publicação: 2023 Tipo de documento: Article