A spectral decomposition quality assessment tool for multi-wavelength AUC experiments with UltraScan.
Eur Biophys J
; 52(4-5): 303-310, 2023 Jul.
Article
em En
| MEDLINE
| ID: mdl-36930298
ABSTRACT
Multi-wavelength analytical ultracentrifugation (MW-AUC) is a recently developed technique that has proven to be a promising tool to investigate mixtures of molecules containing multiple chromophores. It provides an orthogonal separation approach by distinguishing molecules based on their spectral and hydrodynamic properties. Existing software implementations do not permit the user to assess the integrity of the spectral decomposition. To address this shortcoming, we developed a new spectral decomposition residual visualization module, which monitors the accuracy of the spectral decomposition. This module assists the user by providing visual and statistical feedback from the decomposition. The software has been integrated into the UltraScan software suite and an example of a mixture containing thyroglobulin and DNA is presented for illustration purposes.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Software
/
Hidrodinâmica
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Eur Biophys J
Ano de publicação:
2023
Tipo de documento:
Article