Introduction of Genome Editing Reagents and Genotyping of Derived Edited Alleles in Soybean (Glycine max (L.) Merr.).

Quach, Truyen; Nguyen, Hanh; Meyer, Olivia; Sato, Shirley J; Clemente, Tom Elmo; Guo, Ming

Quach, Truyen; Nguyen, Hanh; Meyer, Olivia; Sato, Shirley J; Clemente, Tom Elmo; Guo, Ming.

Afiliação

Quach T; Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, USA.
Nguyen H; Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, USA.
Meyer O; Department of Agronomy & Horticulture, University of Nebraska-Lincoln, Lincoln, NE, USA.
Sato SJ; Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, USA.
Clemente TE; Department of Agronomy & Horticulture, University of Nebraska-Lincoln, Lincoln, NE, USA.
Guo M; Department of Agronomy & Horticulture, University of Nebraska-Lincoln, Lincoln, NE, USA. mguo2@unl.edu.

Methods Mol Biol ; 2653: 273-285, 2023.

Article em En | MEDLINE | ID: mdl-36995632

ABSTRACT

ABSTRACT

Cas9-based genome editing is a powerful genetic tool for loci specifically targeted for genome modification. This chapter describes up-to-date protocols using Cas9-based genome editing technology, including vector construction with GoldenBraid assembly, Agrobacterium-mediated soybean transformation, and identification of editing in the genome.

Assuntos

Edição de Genes; Glycine max; Edição de Genes/métodos; Glycine max/genética; Sistemas CRISPR-Cas/genética; Alelos; Genótipo

Palavras-chave

Cas9; Genome editing; GoldenBraid assembly; Soybean; Transformation

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Glycine max / Edição de Genes Idioma: En Revista: Methods Mol Biol Ano de publicação: 2023 Tipo de documento: Article

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