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Excess of guide RNA reduces knockin efficiency and drastically increases on-target large deletions.
Chenouard, Vanessa; Leray, Isabelle; Tesson, Laurent; Remy, Severine; Allan, Alasdair; Archer, Daniel; Caulder, Adam; Fortun, Agnès; Bernardeau, Karine; Cherifi, Yacine; Teboul, Lydia; David, Laurent; Anegon, Ignacio.
Afiliação
  • Chenouard V; INSERM, Nantes Université, CHU Nantes, Center for Research in Transplantation and Translational Immunology, UMR 1064, F-44000 Nantes, France.
  • Leray I; genOway, Lyon 69007, France.
  • Tesson L; Nantes Université, CHU Nantes, Inserm, CNRS, BioCore, F-44000 Nantes, France.
  • Remy S; INSERM, Nantes Université, CHU Nantes, Center for Research in Transplantation and Translational Immunology, UMR 1064, F-44000 Nantes, France.
  • Allan A; INSERM, Nantes Université, CHU Nantes, Center for Research in Transplantation and Translational Immunology, UMR 1064, F-44000 Nantes, France.
  • Archer D; Mary Lyon Centre, MRC Harwell Institute, Harwell Oxford, UK.
  • Caulder A; Mary Lyon Centre, MRC Harwell Institute, Harwell Oxford, UK.
  • Fortun A; Mary Lyon Centre, MRC Harwell Institute, Harwell Oxford, UK.
  • Bernardeau K; Nantes Université, CHU Nantes, CNRS, Inserm, BioCore, US16, Plateforme P2R, SFR Bonamy, F-44000 Nantes, France.
  • Cherifi Y; Cibles et Médicaments des Infections et du Cancer, IICiMed, Nantes Université, UR 1155, F-44000 Nantes, France.
  • Teboul L; Nantes Université, CHU Nantes, CNRS, Inserm, BioCore, US16, Plateforme P2R, SFR Bonamy, F-44000 Nantes, France.
  • David L; genOway, Lyon 69007, France.
  • Anegon I; Mary Lyon Centre, MRC Harwell Institute, Harwell Oxford, UK.
iScience ; 26(4): 106399, 2023 Apr 21.
Article em En | MEDLINE | ID: mdl-37034986
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: IScience Ano de publicação: 2023 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: IScience Ano de publicação: 2023 Tipo de documento: Article